{"product_id":"t4-polynucleotide-kinase","title":"T4 Polynucleotide Kinase","description":"\u003ch5\u003eProduct Description\u003c\/h5\u003e\n\u003cp\u003eRoke’s T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5′-hydroxyl terminus of DNA oligonucleotides, generating 5′-phosphorylated DNA suitable for downstream molecular biology applications.\u003c\/p\u003e\n\u003cp\u003eT4 PNK is commonly used to prepare DNA oligonucleotides for ligation, cloning, and adapter addition workflows, enabling efficient incorporation of synthetic DNA into enzymatic reactions. Roke's T4 PNK is supplied at 10,000 U\/mL (10 U\/µL) and includes a 10× reaction buffer to support reliable and reproducible phosphorylation under standard reaction conditions.\u003c\/p\u003e\n\u003cp\u003eThis product includes the following components:\u003c\/p\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eT4 Polynucleotide Kinase\u003c\/td\u003e\n\u003ctd\u003e10 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10× T4 PNK Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e700 mM Tris-HCl (pH 7.6 at 25 °C), 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 50 mM DTT\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch5\u003eTechnical Information\u003c\/h5\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eEnzyme\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eT4 Polynucleotide Kinase (T4 PNK)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSource\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eRecombinantly expressed and purified from \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOptimal Reaction Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e37 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReaction Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e70 mM Tris-HCl (pH 7.6 at 25 °C), 10 mM MgCl\u003csub\u003e2\u003c\/sub\u003e, 5 mM DTT \u003cbr\u003e\u003cspan style=\"font-size: 0.95em;\"\u003e(10× buffer supplied; listed composition is at 1×)\u003c\/span\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 mM Tris-HCl (pH 7.4 at 25 °C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 µM ATP, 50% glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eConcentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 U\/µL (10,000 U\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUnit Definition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOne unit (U) catalyzes the phosphorylation of 20 pmol DNA oligonucleotide in 30 minutes at 37 °C (1:1 stoichiometric ATP→ADP conversion).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage Temperature\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e-20 °C (avoid repeated freeze-thaw cycles)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e5′ phosphorylation of DNA oligonucleotides for ligation, cloning, and adapter addition workflows\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct Manual\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0570\/2536\/4154\/files\/T4PNK_manual.docx.pdf?v=1769635115\" target=\"_blank\"\u003eClick here\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCertificate of Analysis\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0570\/2536\/4154\/files\/T4PNK_CoA.docx.pdf?v=1769635175\" target=\"_blank\"\u003eClick here\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Roke Biotechnologies","offers":[{"title":"2000 Units","offer_id":45737812492474,"sku":"RE-PNK2kU","price":74.99,"currency_code":"USD","in_stock":true},{"title":"10000 Units","offer_id":45737812525242,"sku":"RE-PNK10kU","price":299.99,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0570\/2536\/4154\/files\/T4PNK_product.png?v=1774647522","url":"https:\/\/reagents.rokebio.com\/products\/t4-polynucleotide-kinase","provider":"Roke Biotechnologies","version":"1.0","type":"link"}