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Product Description
Roke’s high fidelity 2X PCR Master Mix is a ready-to-use, optimized solutions designed for high-fidelity PCR applications. This master mix contain all necessary components for PCR, except for the DNA template and primers. The composition of 1x Master Mix is Pfu Fusion DNA polymerase (20 units/ml), 0.2 mM dNTPs, 10 mM Tris-HCl (pH 8.5), 50 mM KCl, 1.5 mM MgCl2, 10 mM (NH4)2SO4, 5% glycerol, 0.1% Triton X-100.
Price Comparison
Fusion combines the accuracy of top-tier high-fidelity polymerases with unmatched affordability. Screen once, clone once, and save up to fivefold per reaction without changing your workflow.
What this means for cloning
High-fidelity enzymes keep your downstream work simple. Fusion, Platinum SuperFi, and Q5 require screening just one colony across 1–10 kb inserts, while Taq often needs multiple colonies as insert length grows. The table below shows the estimated number of colonies to pick for a correct clone.
| Polymerase | Error Fold relative to Taq |
Insert Length (bp) — Colonies to Screen (95% confidence) | ||||
|---|---|---|---|---|---|---|
| 1000 | 2000 | 3000 | 5000 | 10000 | ||
| Fusion | 30 | 1 | 1 | 1 | 1 | 1 |
| Platinum SuperFi | 325 | 1 | 1 | 1 | 1 | 1 |
| Q5 | 280 | 1 | 1 | 1 | 1 | 1 |
| Taq | 1 | 3 | 4 | 6 | 11 | 48 |
Assumes independence and binomial sampling; colonies shown are the expected number to reach 95% confidence in an error-free insert.
Technical Information
| Product Manual | Click here |
| Certificate of Analysis | Click here |
| Key References | Menacho-Melgar, R., Yang T, and Lynch, MD., Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase. biorxiv., 25 September 2021. |